Post-resistance exercise photobiomodulation therapy has a more effective antioxidant effect than pre-application on muscle oxidative stress

This study evaluated the effect of photobiomodulation therapy (PBMt) before or after a high-intensity resistance exercise (RE) session on muscle oxidative stress. Female Wistar rats were assigned to one following groups: Sham (non-exercised, undergoing placebo-PBMt); NLRE (exercised, PBMt + RE (pre-exercise PBMt); (post-exercise PBMt). The comprised four climbs bearing maximum load with 2 min rest between each climb. An 830-nm aluminum gallium arsenide diode laser (100 mW; 0.028 cm2; 3.57 mW/cm2; 142.8 J/cm2; 4 J; Photon Laser III, DMC, São Paulo, Brazil) was applied 60 s in gastrocnemius muscles. Analyses performed at 24 h RE: lipoperoxidation using malondialdehyde (MDA) and protein oxidation (OP) Western blot. Superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPx) activity spectrophotometrically assessed. Nitric oxide (NO) level determined by Griess reaction. MDA OP levels significantly higher group. Increased prevented all groups; however, increased only PBMT group had SOD compared other groups. A GPx observed group, CAT reduced RE, without effect. NO unchanged PBMt. Therefore, application section has more potent antioxidant than previous Rats submitted post-RE illustrated prevention as well activity. can attenuate stress induced exercise. evident benefit shows be obtained exercise, which it superoxide dismustase.